Contact an Account ManagerEquivalence of a HPLC assay and a bioassay of azithromycin in human serum samples
Summary
Type: HPLC
Application #: 103600
Catalog#: 720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection: electrochemical, guard cell at +1V, dual electrode cell at +0.7 and +0.8V
Conditions: eluent: 0.04M Na2HPO4 - 0.005M tetrabutylammonium perchlorate - acetonitrile (50:50:33, v/v), adjusted to pH 7 with 25% phosphoric acid
flow rate 1ml/min
Authors: Riedel K-D
Source: J Chromatogr 576 (1992) 358 - 362
Details
Legend: Fig. 1. HPLC of extracts of A) control human serum, B) serum collected from a
subject 2.5h after a single 600mg dose of azithromycin, containing 0.27µg/ml
azithromycin.